PeakTrace Basecaller
The PeakTrace Basecaller™ is a Sanger DNA sequencing basecaller and trace processor for improving the readlength and quality of ABI sequencing traces, developed by Nucleics.
The PeakTrace Basecaller provides from 15% to 100% more high quality bases as compared to basecallers such as ABI's KB Basecaller™ or phred. In addition to offering more data, it improves the DNA peak resolution, making it easier to manually check the basecall.
PeakTrace is able to basecall DNA sequencing trace files collected by the ABI 377, ABI 310, ABI 3700, ABI 3100, ABI 3130, ABI 3500, ABI 3730, ABI 3730xl, ABI 3200 (SeqStudio), ABI 3600 (SeqStudio Flex), MegaBACE 1000 and MegaBACE 4000 DNA sequencers.
The images below show a typical example obtained from a 50cm ABI 3730 trace file. The left image was basecalled with PeakTrace and the right is the original ABI KB basecaller trace. This is the same trace file basecalled with the two different basecallers.
You can trial PeakTrace for free on your own traces by visiting the PeakTrace Online page. For further information on PeakTrace, please download our PeakTrace Whitepaper.
Auto PeakTrace RP
Auto PeakTrace RP is a user installable version of the Auto PeakTrace software which allows users to upload their traces to the PeakTrace server using the Auto PeakTrace RP software and have them processed automatically. You can find out more about PeakTrace RP by visiting the PeakTrace DNA basecaller information page or the PeakTrace FAQ page. The latest release of Auto PeakTrace RP is 6.961 (16 Jun 2023).
